2GV9
Crystal structure of the Herpes Simplex virus type 1 DNA polymerase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-11-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0, 0.9794, 0.9504 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 103.917, 125.550, 220.577 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.300 - 2.680 |
| R-factor | 0.2248 |
| Rwork | 0.222 |
| R-free | 0.28057 |
| Structure solution method | MAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.815 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.300 | 2.800 |
| High resolution limit [Å] | 2.675 | 2.675 |
| Rmerge | 0.071 | 0.849 |
| Number of reflections | 81295 | |
| <I/σ(I)> | 30.2 | 2.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.1 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 100mM HEPES, 50mM ammonium sulphate, 5mM DTT, 100mM guanidine-HCl, 4% PEG 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP |
