2GUU
crystal structure of Plasmodium vivax orotidine 5-monophosphate decarboxylase with 6-aza-UMP bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-04-05 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 69.731, 82.151, 59.739 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.090 - 1.860 |
| R-factor | 0.1899 |
| Rwork | 0.188 |
| R-free | 0.23614 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ffc |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.456 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (V. 1.3.1) |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.090 | 1.890 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.100 | 0.823 |
| Number of reflections | 27267 | |
| <I/σ(I)> | 9.8 | 1.57 |
| Completeness [%] | 91.8 | 54 |
| Redundancy | 7 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 297 | 30% PEG 4K, 0.2 M Sodium Acetate, 0.1M Tris HCl, pH 8.0, VAPOR DIFFUSION, temperature 297K |
