2GUM
Crystal structure of the extracellular domain of glycoprotein B from Herpes Simplex Virus type I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97944 |
| Spacegroup name | P 1 |
| Unit cell lengths | 84.959, 100.039, 100.133 |
| Unit cell angles | 67.04, 77.99, 70.31 |
Refinement procedure
| Resolution | 41.200 - 2.100 |
| R-factor | 0.2479 |
| Rwork | 0.248 |
| R-free | 0.27470 |
| Structure solution method | SAD |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.200 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.066 | 0.331 |
| Number of reflections | 145731 | |
| <I/σ(I)> | 18.1 | 3.7 |
| Completeness [%] | 0.9 | 0.588 |
| Redundancy | 4.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 8% PEG 6000, 0.1 M Na Hepes, 15% 2-methyl-2,4-pentanediol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
