2GUD
Crystal structure of a complex of griffithsin with mannose at 0.94 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-26 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.7 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.740, 39.440, 57.160 |
| Unit cell angles | 90.00, 91.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 0.940 |
| R-factor | 0.1355 |
| Rwork | 0.136 |
| R-free | 0.15607 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gty |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.154 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 0.960 |
| High resolution limit [Å] | 0.940 | 0.940 |
| Number of reflections | 148669 | |
| Completeness [%] | 95.5 | 75 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 1.8M magnesium sulfate,0.1M MES, 1:10 ratio of griffithsin monomers to mannose, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
