2GTP

Crystal structure of the heterodimeric complex of human RGS1 and activated Gi alpha 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2006-03-04
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9789
Spacegroup name	P 21 21 21
Unit cell lengths	90.451, 102.904, 128.761
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.700 - 2.550
R-factor	0.22839
Rwork	0.226
R-free	0.27562
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB IDs 2BV1 and 1Y3A used as starting models for RGS1 and GNAI1 respectively.
RMSD bond length	0.012
RMSD bond angle	1.354
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.640
High resolution limit [Å]	2.550	2.550
Number of reflections	39406
Completeness [%]	98.5	99

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.2M NaNO3, 0.1M bis-Tris propane, pH 6.5, 20 % PEG3350, 10 % Ethylene glycol, VAPOR DIFFUSION, SITTING DROP, temperature 293K