2GTL
Lumbricus Erythrocruorin at 3.5A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-01-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 |
| Unit cell lengths | 176.080, 257.960, 436.530 |
| Unit cell angles | 89.69, 97.15, 90.98 |
Refinement procedure
| Resolution | 100.000 - 3.500 |
| R-factor | 0.288 |
| Rwork | 0.288 |
| R-free | 0.29700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 3.630 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.078 | |
| Number of reflections | 890862 | |
| Completeness [%] | 89.3 | 74.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 298 | 6% (w/w) PEG 8000, 10mM CaCl2, 3% isopropanol, 60mM Hepes, pH 7.5, VAPOR DIFFUSION, temperature 298.0K |
