2GTD
Crystal Structure of a Type III Pantothenate Kinase: Insight into the Catalysis of an Essential Coenzyme A Biosynthetic Enzyme Universally Distributed in Bacteria
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Collection date | 2005-12-14 |
| Wavelength(s) | 0.97872, 0.97886 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.112, 137.788, 75.221 |
| Unit cell angles | 90.00, 109.23, 90.00 |
Refinement procedure
| Resolution | 31.560 - 2.000 |
| R-factor | 0.188 |
| Rwork | 0.188 |
| R-free | 0.24500 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.431 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.560 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 95595 | |
| Completeness [%] | 97.7 | 97.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 15% PEG-3350, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
