2GSJ
cDNA cloning and 1.75A crystal structure determination of PPL2, a novel chimerolectin from Parkia platycephala seeds exhibiting endochitinolytic activity
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-06-01 |
| Detector | MAR scanner 180 mm plate |
| Wavelength(s) | 1.43 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.190, 59.949, 76.697 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.310 - 1.730 |
| R-factor | 0.1749 |
| Rwork | 0.169 |
| R-free | 0.19873 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.485 |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.310 | 1.830 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Rmerge | 0.040 | 0.228 |
| Number of reflections | 25805 | |
| <I/σ(I)> | 20.8 | 8.1 |
| Completeness [%] | 27.0 | |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 0.2 M ammonium acetate, 0,1 M trisodium citrate dehydrate, pH 5.6 and 30% (w/v) PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
