2GSH
Crystal structure of L-rhamnonate dehydratase from Salmonella typhimurium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-16 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 2 3 |
| Unit cell lengths | 135.145, 135.145, 135.145 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.393 |
| R-factor | 0.19553 |
| Rwork | 0.194 |
| R-free | 0.24084 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gl5 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.235 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.000 | 2.520 |
| High resolution limit [Å] | 2.393 | 2.393 |
| Rmerge | 0.085 | 0.562 |
| Number of reflections | 32489 | |
| <I/σ(I)> | 7.6 | 0.9 |
| Completeness [%] | 99.6 | 97.2 |
| Redundancy | 20.3 | 15.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 290 | 2.4M SODIUM MALONATE, 10% GLYCEROL, pH 7.00, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
