2GSC

Crystal Structure of the Conserved Hypothetical Cytosolic Protein Xcc0516 from Xanthomonas campestris

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL12B2
Synchrotron site	SPring-8
Beamline	BL12B2
Temperature [K]	100
Detector technology	CCD
Collection date	2005-06-20
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9537, 0.97991, 0.97972
Spacegroup name	P 21 21 2
Unit cell lengths	87.794, 104.257, 66.489
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.170 - 2.450
R-factor	0.22
Rwork	0.220
R-free	0.28300
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.246
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.540
High resolution limit [Å]	2.450	2.450
Rmerge	0.058	0.146
Number of reflections	22715
<I/σ(I)>	16.7
Completeness [%]	98.5	98.9
Redundancy	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	277	200mM KCl, 5mM MgCl2, 50mM Na cacodylate pH 6.0, 16%(w/v) 1,6-hexanediol, 100mM CsCl, VAPOR DIFFUSION, HANGING DROP, temperature 277K