2GRZ
5ns Photoproduct of the M37V mutant of Scapharca HbI
Experimental procedure
| Experimental method | LAUE |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 283 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-04-07 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.0-1.4 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 93.040, 43.930, 83.440 |
| Unit cell angles | 90.00, 121.95, 90.00 |
Refinement procedure
| Resolution | 16.850 - 1.600 |
| R-factor | 0.106 |
| Rwork | 0.106 |
| R-free | 0.10900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2grh |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Data reduction software | Precognition |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.670 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 28081 | |
| Completeness [%] | 73.8 | 33.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SMALL TUBES | 7.5 | 298 | 57.5% Saturated Phosphate buffer, SMALL TUBES, temperature 298K |
