2GR7
Hia 992-1098
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Wavelength(s) | 0.931 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 97.340, 56.296, 158.706 |
| Unit cell angles | 90.00, 90.04, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.226 |
| Rwork | 0.226 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.401 |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.300 |
| Number of reflections | 38510 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 10 | 293 | 100 mM CHES, 12% PEG 4000 and 200 mM ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
