2GPC
The crystal structure of the enzyme Fe-superoxide dismutase from Trypanosoma cruzi
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2002-02-25 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 109.626, 38.985, 91.073 |
Unit cell angles | 90.00, 105.85, 90.00 |
Refinement procedure
Resolution | 29.490 - 1.900 |
R-factor | 0.15666 |
Rwork | 0.154 |
R-free | 0.19810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1isa |
RMSD bond length | 0.008 |
RMSD bond angle | 1.118 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.500 | 1.990 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 27127 | |
<I/σ(I)> | 10.6 | 2.7 |
Completeness [%] | 94.6 | 69.9 |
Redundancy | 4.4 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 24% PEG4000, 0.2 M MgCl2, 0.1 M TRIS-HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |