2GPA
ALLOSTERIC INHIBITION OF GLYCOGEN PHOSPHORYLASE A BY A POTENTIAL ANTIDIABETIC DRUG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-07 |
| Detector | MAR scanner 180 mm plate |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 127.200, 127.200, 116.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.000 |
| R-factor | 0.179 * |
| Rwork | 0.179 |
| R-free | 0.23000 |
| Structure solution method | OTHER |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 13.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.079 | 0.410 |
| Total number of observations | 448708 * | |
| Number of reflections | 57879 | |
| <I/σ(I)> | 14.6 | 5 |
| Completeness [%] | 90.1 | 92.6 |
| Redundancy | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | other * | 6.7 | Fletterick, R.J., (1976) J. Mol. Biol., 103, 1. * |
