2GOX
Crystal structure of Efb-C / C3d Complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2005-11-21 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.9184 |
Spacegroup name | P 41 |
Unit cell lengths | 90.939, 90.939, 122.242 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.200 |
Rwork | 0.181 |
R-free | 0.23100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1c3d |
RMSD bond length | 0.024 |
RMSD bond angle | 1.877 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.110 | 0.532 |
Number of reflections | 49761 | 4664 |
<I/σ(I)> | 9 | 1.8 |
Completeness [%] | 99.0 | 92.4 |
Redundancy | 4.9 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 60% Tacsimate pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |