2GOM
Crystal structure of Efb-C from Staphylococcus aureus
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2005-08-12 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97949, 0.97934, 0.97178 |
| Spacegroup name | P 43 |
| Unit cell lengths | 59.598, 59.598, 45.634 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 59.550 - 1.250 |
| R-factor | 0.21095 |
| Rwork | 0.211 |
| R-free | 0.21804 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.928 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.240 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.063 | 0.544 |
| Number of reflections | 44291 | |
| <I/σ(I)> | 16.7 | 2.2 |
| Completeness [%] | 86.9 | 99.2 |
| Redundancy | 3.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 3M Sodium Acetate pH 7.4 , VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
