2GO1
NAD-dependent formate dehydrogenase from Pseudomonas sp.101
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-15 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.009 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 93.300, 93.300, 103.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 69.010 - 2.100 |
| R-factor | 0.18923 |
| Rwork | 0.186 |
| R-free | 0.24224 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nac |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.737 |
| Data reduction software | MARHKL |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.010 | 2.130 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.078 | 0.495 |
| Number of reflections | 29590 | |
| Completeness [%] | 94.7 | 91.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 290 | Ammonium Sulfate (48% saturated), 8% MPD, 0.1M HEPES, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
