2GNP
Structural Genomics, the crystal structure of a transcriptional regulator from Streptococcus pneumoniae TIGR4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97907 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 107.044, 34.451, 71.627 |
| Unit cell angles | 90.00, 101.53, 90.00 |
Refinement procedure
| Resolution | 30.400 - 1.650 |
| R-factor | 0.1956 |
| Rwork | 0.193 |
| R-free | 0.23677 |
| Structure solution method | SAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.782 |
| Data reduction software | HKLSBC |
| Data scaling software | HKL-2000 |
| Phasing software | HKLSBC |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.400 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.078 | 0.528 |
| Number of reflections | 30578 | |
| <I/σ(I)> | 30.39 | 2.57 |
| Completeness [%] | 97.7 | 98.7 |
| Redundancy | 4.6 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.1M Sodium Acetate, 0.1M MES, 30% PEG 400, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
