2GNO

Crystal structure of a dna polymerase iii, gamma subunit-related protein (tm0771) from thermotoga maritima msb8 at 2.00 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-12-18
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979224, 0.918370, 0.978940
Spacegroup name	I 4
Unit cell lengths	135.560, 135.560, 35.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.940 - 2.000
R-factor	0.223
Rwork	0.220
R-free	0.27800
Structure solution method	MAD
RMSD bond length	0.013
RMSD bond angle	1.684
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHARP (V. 1)
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	27.900		2.070
High resolution limit [Å]	2.000	3.420	2.000
Rmerge	0.041	0.025	0.415
Number of reflections	22105	4199	3580
<I/σ(I)>	11.88	25.1	2
Completeness [%]	94.7	99.1	83.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP, NANODROP	6.2	277	35.0% 2-ethoxyethanol, 0.2M NaCl, 0.1M Na,K-Phosphate, pH 6.2, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 277K