2GMI

Mms2/Ubc13~Ubiquitin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2005-09-25
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0039
Spacegroup name	P 21 21 21
Unit cell lengths	53.230, 86.410, 86.620
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.500
Rwork	0.237
R-free	0.26800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jat 1ubq
RMSD bond length	0.007
RMSD bond angle	1.496
Data scaling software	HKL-2000
Phasing software	EPMR
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.500	2.500
Number of reflections	14242
<I/σ(I)>	44	3.1
Completeness [%]	99.6	96.9
Redundancy	6.8	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	277	17% PEG 1000, 100 mM Na/K phosphate, pH 6.2, 25 mM NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 277K