2GM9
Structure of rabbit muscle glycogen phosphorylase in complex with thienopyrrole
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-10-07 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.93 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 126.781, 126.781, 115.144 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 91.290 - 2.300 |
| R-factor | 0.18564 |
| Rwork | 0.182 |
| R-free | 0.24510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | IN HOUSE MODEL |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.566 |
| Data reduction software | MOSFLM |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 91.290 | 2.540 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 39447 | |
| Completeness [%] | 98.3 | 39.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 289 | 1.1mM IMP 1.1mM Spermin 10mM BES 2.9mM DTT 0.1mM EDTA, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
