2GM5
An activated, truncated gamma-delta resolvase tetramer
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97180, 0.97930, 0.97950 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.331, 98.476, 100.408 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.140 - 2.100 |
| R-factor | 0.219 |
| Rwork | 0.215 |
| R-free | 0.25500 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.947 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.089 | 0.738 |
| Number of reflections | 59517 | 5922 |
| <I/σ(I)> | 7.7 | 1.9 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | Protein in 200 mM NaCl, 3.75 mM DTT, 40 mM Tris-HCl, 0.05 M Li2SO4, 7.5% PEG 4000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
