2GLU
The crystal structure of YcgJ protein from Bacillus subitilis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-11-04 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | .979 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 69.899, 73.639, 245.742 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.910 |
R-factor | 0.19686 |
Rwork | 0.192 |
R-free | 0.28257 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xxl |
RMSD bond length | 0.017 |
RMSD bond angle | 1.648 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0016) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 13553 | |
Completeness [%] | 94.6 | 96.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 2.4M Am Sulfate, 0.1M Na Citrate , pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |