2GLK
High-resolution study of D-Xylose isomerase, 0.94A resolution.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-09-08 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.0 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 92.712, 97.935, 101.867 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.360 - 0.940 |
R-factor | 0.1157 |
Rwork | 0.115 |
R-free | 0.12792 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xib |
RMSD bond length | 0.021 |
RMSD bond angle | 1.917 |
Data reduction software | X-GEN |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.360 | 0.965 |
High resolution limit [Å] | 0.940 | 0.944 |
Number of reflections | 252079 | |
Completeness [%] | 85.6 | 12 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8 | 293 | 50mM tris-HCl,25%AMSO4,2mM Mn2+ and 2mM Co2+ , XI @ 25mg/ml., pH 8, VAPOR DIFFUSION, temperature 293K |