2GK9
Human Phosphatidylinositol-4-phosphate 5-kinase, type II, gamma
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-25 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.953 |
| Spacegroup name | P 43 |
| Unit cell lengths | 95.390, 95.390, 189.110 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.800 |
| R-factor | 0.261 |
| Rwork | 0.254 |
| R-free | 0.29700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.663 | |
| Number of reflections | 40823 | |
| <I/σ(I)> | 8.23 | 2.13 |
| Completeness [%] | 98.3 | 99.7 |
| Redundancy | 2.55 | 3.18 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 273 | 20 % Peg3350 0.3 M di-ammoniumtartrate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 273K |
