2GK6
Structural and Functional insights into the human Upf1 helicase core
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-20 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 |
| Unit cell lengths | 63.284, 67.836, 87.250 |
| Unit cell angles | 114.41, 90.13, 110.22 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.23709 |
| Rwork | 0.235 |
| R-free | 0.27193 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gjk |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.126 |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.400 |
| Number of reflections | 43423 |
| Completeness [%] | 90.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7.5 | 295 | 100mM Tris, 8-13% PEG3350, 140mM (NH4)3PO4, 10% EG, 10mM DTT, pH 7.5, EVAPORATION, temperature 295K |
