2GJW
RNA Recognition and Cleavage by an Splicing Endonuclease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 8-BM |
| Synchrotron site | APS |
| Beamline | 8-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 90.073, 140.357, 82.048 |
| Unit cell angles | 90.00, 111.69, 90.00 |
Refinement procedure
| Resolution | 35.090 - 2.850 |
| R-factor | 0.24986 |
| Rwork | 0.247 |
| R-free | 0.29646 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.831 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.090 | 2.920 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Number of reflections | 40048 | |
| Completeness [%] | 90.2 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 303 | VAPOR DIFFUSION, HANGING DROP, PH 6.5, 303K, 0.12M AMMONIUM ACETATE, 35mM MAGNESIUM ACETATE, 50mM SODIUM CACODYLATE,10% ISOPROPANOL |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | AMMONIUM ACETATE | ||
| 2 | 1 | 1 | MAGNESIUM ACETATE | ||
| 3 | 1 | 1 | SODIUM CACODYLATE | ||
| 4 | 1 | 1 | ISOPROPANOL | ||
| 5 | 1 | 1 | H2O | ||
| 6 | 1 | 2 | AMMONIUM ACETATE | ||
| 7 | 1 | 2 | MAGNESIUM ACETATE | ||
| 8 | 1 | 2 | SODIUM CACODYLATE |
