2GJE
Structure of a guideRNA-binding protein complex bound to a gRNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.989 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 157.600, 157.600, 81.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 69.850 - 3.370 |
| R-factor | 0.2821 |
| Rwork | 0.282 |
| R-free | 0.30000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.400 |
| Data scaling software | SCALA |
| Phasing software | EPMR |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.600 | 3.450 |
| High resolution limit [Å] | 3.370 | 3.370 |
| Number of reflections | 8126 | |
| Completeness [%] | 92.0 | 70 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 280 | peg 4000, hepes, isopropanol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 280K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | peg 4000 | ||
| 2 | 1 | 1 | hepes | ||
| 3 | 1 | 1 | isopropanol | ||
| 4 | 1 | 2 | peg 4000 | ||
| 5 | 1 | 2 | hepes | ||
| 6 | 1 | 2 | isopropanol |
