2GJD
Distinct functional domains of Ubc9 dictate cell survival and resistance to genotoxic stress
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-01-30 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.100 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 59.304, 60.141, 95.287 |
Unit cell angles | 90.00, 105.01, 90.00 |
Refinement procedure
Resolution | 25.000 - 1.750 |
R-factor | 0.21 |
Rwork | 0.210 |
R-free | 0.23000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1u9a |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.810 |
High resolution limit [Å] | 1.750 | 1.750 |
Number of reflections | 65226 | |
Completeness [%] | 99.5 | 98.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 18% PEG 6000, 0.1M HEPES, 0.1M NaBr, 5mM DTT, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |