2GJA
Structure of the MnmE G-domain in complex with GDP*AlF4-, Mg2+ and NH4+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Detector technology | CCD |
| Collection date | 2005-04-18 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.950 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 71.280, 90.650, 138.470 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.370 - 1.850 |
| R-factor | 0.19688 |
| Rwork | 0.195 |
| R-free | 0.22837 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.498 |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.900 | |
| High resolution limit [Å] | 1.850 | 6.000 | 1.850 |
| Rmerge | 0.068 | 0.037 | 0.317 |
| Number of reflections | 38156 | ||
| <I/σ(I)> | 14.44 | 29.5 | 5.5 |
| Completeness [%] | 97.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1.5 M ammonium sulphate, 100 mM HEPES 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
