2GJ8
Structure of the MnmE G-domain in complex with GDP*AlF4-, Mg2+ and K+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Detector technology | CCD |
| Collection date | 2005-12-03 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.540, 70.227, 91.300 |
| Unit cell angles | 90.00, 95.51, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| R-factor | 0.20766 |
| Rwork | 0.205 |
| R-free | 0.25563 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.568 |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.800 | |
| High resolution limit [Å] | 1.700 | 6.000 | 1.700 |
| Rmerge | 0.081 | 0.043 | 0.312 |
| Number of reflections | 79655 | ||
| <I/σ(I)> | 8.47 | 19.7 | 3.8 |
| Completeness [%] | 97.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 16-19% PEG 3350, 100 mM MES pH 6.0, 200 mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
