2GIS
Structure of the S-adenosylmethionine riboswitch mRNA regulatory element
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-11-11 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.10532, 1.10573 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 62.901, 62.901, 158.967 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.320 - 2.900 |
R-factor | 0.266 |
Rwork | 0.266 |
R-free | 0.28900 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.600 |
Data scaling software | d*TREK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.320 | 3.080 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.072 | 0.426 |
Number of reflections | 14940 | |
<I/σ(I)> | 17.9 | 4.4 |
Completeness [%] | 99.6 | 99.9 |
Redundancy | 14.64 | 11.74 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |