2GIM
1.6 Angstrom structure of plastocyanin from Anabaena variabilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-25 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.11 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.850, 45.810, 63.410 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.190 - 1.600 |
| R-factor | 0.21138 |
| Rwork | 0.209 |
| R-free | 0.26021 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | : 1BAW |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.859 |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.800 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Number of reflections | 24943 | |
| Completeness [%] | 93.3 | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 300 | 1.15 M trisodium citrate, 7.67 mM sodium borate buffer pH 8.5 , VAPOR DIFFUSION, SITTING DROP, temperature 300K |
