2GIL
Structure of the extremely slow GTPase Rab6A in the GTP bound form at 1.8 resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0398 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.008, 124.853, 66.162 |
| Unit cell angles | 90.00, 107.57, 90.00 |
Refinement procedure
| Resolution | 19.050 - 1.820 |
| R-factor | 0.20849 |
| Rwork | 0.205 |
| R-free | 0.27735 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1d5c |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.400 |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.050 | 1.900 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.056 | 0.249 |
| Number of reflections | 48538 | |
| <I/σ(I)> | 17.44 | 4.3 |
| Completeness [%] | 87.7 | 52.2 |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 100mM TrisHCl, 1M LiCl, 32% (w/v) polyethylene glycol 1000, 3.3mM Nonyl-D-glucoside, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
