2GI3

Crystal structure of Glutamyl-tRNA(Gln) amidotransferase subunit A (tm1272) from THERMOTOGA MARITIMA at 1.80 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL1-5
Synchrotron site	SSRL
Beamline	BL1-5
Temperature [K]	100
Detector technology	CCD
Collection date	2006-01-23
Detector	ADSC QUANTUM 315
Wavelength(s)	0.918381, 0.978662
Spacegroup name	P 32 2 1
Unit cell lengths	131.776, 131.776, 61.987
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.910 - 1.800
R-factor	0.17628
Rwork	0.175
R-free	0.20100
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.490
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.910	29.910	1.850
High resolution limit [Å]	1.800	8.050	1.800
Rmerge	0.155	0.048	0.729
Number of reflections	57320
<I/σ(I)>	4.5	12.3	1
Completeness [%]	99.8		98.3
Redundancy	6.9	6.9	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.6	277	0.02M CaCl2, 30.0% MPD, 0.1M Acetate pH 4.6 , VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K