2GHY

Novel Crystal Form of the ColE1 Rom Protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-08-01
Detector	RIGAKU RAXIS II
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	97.770, 39.110, 26.700
Unit cell angles	90.00, 96.16, 90.00

Refinement procedure

Resolution	20.000 - 2.500
R-factor	0.179
Rwork	0.179
R-free	0.26300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gto
RMSD bond length	0.008
RMSD bond angle	2.151
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.610
High resolution limit [Å]	2.500	2.500
Number of reflections	3487
Completeness [%]	97.5	96.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	295	30% PEG MME 2000, 0.1M sodium acetate, pH4.6, and 0.2M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 295K