2GGL
The mutant A222C of Agrobacterium radiobacter N-carbamoyl-D-amino acid amidohydrolase
Replaces: 2FKUReplaces: 2BA4Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-04-13 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.379, 68.101, 138.191 |
| Unit cell angles | 90.00, 96.09, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.400 |
| R-factor | 0.16975 |
| Rwork | 0.168 |
| R-free | 0.20832 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fo6 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.381 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 50489 | |
| Completeness [%] | 94.8 | 93.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | lithium sulfate, HEPES buffer, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
