2GFQ
Structure of Protein of Unknown Function PH0006 from Pyrococcus horikoshii
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.021, 67.029, 111.572 |
| Unit cell angles | 90.00, 90.09, 90.00 |
Refinement procedure
| Resolution | 35.800 - 1.750 |
| R-factor | 0.15193 |
| Rwork | 0.150 |
| R-free | 0.17904 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.302 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.800 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.047 | 0.194 |
| Number of reflections | 91292 | |
| <I/σ(I)> | 10.2 | 4.2 |
| Completeness [%] | 94.2 | 94.8 |
| Redundancy | 2.4 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 2.3M ammonium sulfate, 2% PEG MME 2K, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
