2GF2
Crystal structure of human hydroxyisobutyrate dehydrogenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-04 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97640 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.053, 122.745, 200.397 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.560 - 2.380 |
| R-factor | 0.2073 |
| Rwork | 0.205 |
| R-free | 0.24677 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.131 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.560 | 2.470 |
| High resolution limit [Å] | 2.380 | 2.380 |
| Rmerge | 0.140 | 0.526 |
| Number of reflections | 50370 | |
| Completeness [%] | 99.5 | 98.5 |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 0.20M LiCl, 0.1M HEPES, 20.0% PEG 6K, 10.0% Ethylen Glycol, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
