2GF0
The crystal structure of the human DiRas1 GTPase in the inactive GDP bound state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-04 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9789 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.053, 94.031, 61.214 |
| Unit cell angles | 90.00, 89.98, 90.00 |
Refinement procedure
| Resolution | 47.090 - 1.900 |
| R-factor | 0.16128 |
| Rwork | 0.160 |
| R-free | 0.20781 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2erx |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.398 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 55909 | |
| Completeness [%] | 97.7 | 99.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 3.5 M Sodium formate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
