2GEW
Atomic resolution structure of cholesterol oxidase @ pH 9.0 (Streptomyces sp. SA-COO)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-01-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.110, 73.256, 62.432 |
| Unit cell angles | 90.00, 104.05, 90.00 |
Refinement procedure
| Resolution | 31.300 - 0.970 |
| R-factor | 0.113 |
| Rwork | 0.113 |
| R-free | 0.14000 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1mxt |
| RMSD bond length | 0.015 |
| RMSD bond angle | 0.033 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | SHELXL |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.300 | 1.000 |
| High resolution limit [Å] | 0.970 | 0.970 |
| Rmerge | 0.075 | 0.510 |
| Number of reflections | 243484 | |
| <I/σ(I)> | 11.8 | 1.9 |
| Completeness [%] | 93.0 | 79 |
| Redundancy | 4.5 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 298 | PEG 8000, AMMONIUM SULFATE, PIPES PH 7.5,VAPOR DIFFUSION, HANGING DROP, temperature 298K, SOAK CONDITION STEP 1: PEG 8000, AMMONIUM SULFATE, HEPES PH 8.0 SOAK CONDITION STEP 2: PEG 8000, AMMONIUM SULFATE, TRICINE PH 8.5 SOAK CONDITION STEP 3: PEG 8000, AMMONIUM SULFATE, CHES PH 9.0, |
