2GEE
Crystal Structure of Human Type III Fibronectin Extradomain B and Domain 8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 200 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-01-09 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.407, 55.288, 74.903 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.360 - 2.010 |
| R-factor | 0.249 |
| Rwork | 0.249 |
| R-free | 0.30100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | fragment of Repeat domain 8 from 1fnf |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.900 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.360 | 2.130 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Number of reflections | 12553 | |
| Completeness [%] | 99.6 | 98.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 10% (w/v) PEG-3350 and 0.2 mM ammonium citrate. , pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
