2GE8
Structure of the C-terminal dimerization domain of infectious bronchitis virus nucleocapsid protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 178 |
| Detector technology | CCD |
| Collection date | 2003-12-19 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 108.990, 128.534, 71.435 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.780 - 2.200 |
| R-factor | 0.24 |
| Rwork | 0.237 |
| R-free | 0.29100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2GE7 4 copies |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.919 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 129.473 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.115 | 0.013 |
| Total number of observations | 35383 | |
| Number of reflections | 66258 | 9713 |
| <I/σ(I)> | 4.8 | 0.5 |
| Completeness [%] | 95.8 | |
| Redundancy | 4.4 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 298 | 30% PEG 4000, 100 mM Tris-HCl pH 8.6, 800 mM LiCl, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
