2GE7

Structure of the C-terminal dimerization domain of infectious bronchitis virus nucleocapsid protein

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	178
Detector technology	CCD
Collection date	2004-06-24
Detector	SBC-2
Wavelength(s)	0.97937 , 0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	36.658, 65.707, 91.008
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.450 - 2.000
R-factor	0.193
Rwork	0.190
R-free	0.25400
Structure solution method	MAD
RMSD bond length	0.025
RMSD bond angle	1.970
Data scaling software	SCALA
Phasing software	SnB
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.452	2.000
High resolution limit [Å]	1.984	1.900
Rmerge	0.072	0.282
Number of reflections	15780
<I/σ(I)>	28.8	0.73
Completeness [%]	96.0	95
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.8	298	PEG 4000, 28.75% to 29.5 %, pH 4.8 Citrate, 0.1 M MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K