2GDZ
Crystal structure of 15-hydroxyprostaglandin dehydrogenase type1, complexed with NAD+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 49.540, 49.540, 195.771 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.860 - 1.650 |
| R-factor | 0.17746 |
| Rwork | 0.176 |
| R-free | 0.21214 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wmb 1iy8 1zk3 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.627 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 196.120 | 1.740 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.077 | 0.688 |
| Number of reflections | 30422 | |
| <I/σ(I)> | 23.9 | 2.5 |
| Completeness [%] | 99.6 | 99.6 |
| Redundancy | 11.9 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 0.1 PCB, 30% PEG 1000, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
