2GCY
humanized antibody C25 Fab fragment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-08-30 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.115870 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 90.260, 90.260, 217.300 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.600 - 2.500 |
R-factor | 0.225 |
Rwork | 0.225 |
R-free | 0.23200 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.400 |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 44.600 |
High resolution limit [Å] | 2.500 |
Number of reflections | 17714 |
Completeness [%] | 93.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 298 | PEG MME 550, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K |