2GCT

STRUCTURE OF GAMMA-CHYMOTRYPSIN IN THE RANGE PH 2.0 TO PH 10.5 SUGGESTS THAT GAMMA-CHYMOTRYPSIN IS A COVALENT ACYL-ENZYME ADDUCT AT LOW PH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	285
Detector technology	FILM
Detector	KODAK
Spacegroup name	P 42 21 2
Unit cell lengths	69.800, 69.800, 98.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.800
Rwork	0.169
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.019
RMSD bond angle	18.100 *
Data reduction software	OSCTST
Data scaling software	AGROVATA/ROTAVATE
Refinement software	TNT

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000 *
High resolution limit [Å]	1.800 *
Rmerge	0.068 *
Total number of observations	17029 *
Number of reflections	13431 *
Completeness [%]	63.2 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	7 *		pH 2.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	ammonium sulfate	50 (%sat)