2GC1
The crystal structure of phosphoglucose isomerase from Pyrococcus furiosus in complex with sorbitol 6-phosphate and zinc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-03-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.311, 72.857, 71.640 |
| Unit cell angles | 90.00, 103.57, 90.00 |
Refinement procedure
| Resolution | 19.920 - 1.950 |
| R-factor | 0.201 |
| Rwork | 0.198 |
| R-free | 0.25500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1x8e |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.653 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.673 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.062 | 0.379 |
| Total number of observations | 17653 | |
| Number of reflections | 28380 | 3961 |
| <I/σ(I)> | 9.6 | 2 |
| Completeness [%] | 92.1 | 88.2 |
| Redundancy | 4.6 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 290 | 0.05 M ammonium sulphate, 0.1 M sodium acetate pH 4.6, 20% PEG monoethyl ether 2000, 5 mM D-sorbitol 6-phosphate, 5mM ZnCl2, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
