2GBX
Crystal Structure of Biphenyl 2,3-Dioxygenase from Sphingomonas yanoikuyae B1 Bound to Biphenyl
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 133.940, 133.940, 219.708 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.480 - 2.800 |
| R-factor | 0.238 |
| Rwork | 0.237 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gbw |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.671 |
| Data scaling software | XSCALE |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.481 | 3.000 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.269 | |
| Number of reflections | 56587 | |
| <I/σ(I)> | 6.3 | |
| Completeness [%] | 99.4 | 98.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.8 | 277.15 | 20% PEG3350, 1.0 M Sodium Chloride, 0.037M Zinc Chloride, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K, pH 6.80 |
