2GBV
C6A/C111A/C57A/C146A holo CuZn Superoxide dismutase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-26 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.080 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 166.800, 202.370, 143.640 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.880 - 2.000 |
| R-factor | 0.184 |
| Rwork | 0.183 |
| R-free | 0.20700 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1n18 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.055 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.064 | 0.239 |
| Number of reflections | 162729 | |
| <I/σ(I)> | 8.9 | |
| Completeness [%] | 99.9 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 2.8M Ammonium Sulphate, 50mM NaAc, 0.1M TRIS-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
